FastQCFastQC Report
Wed 25 May 2016
SRR1033249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210449
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76100.038.4112551
GACCGAT658.6888747E-426.5238327
TATGCCG5150.026.11181343
GTATCAA138250.025.4922281
CCGAAAT15850.025.45283947
GCTCTAG9700.023.5095861
TCGTATG5750.023.38737740
TACCGAC902.1354506E-422.98759541
TAGACCG902.1356223E-422.9873225
GCGTTAG902.1356223E-422.98732226
TCGCCCC18050.022.15978438
CGAATTA1103.2075513E-522.00909265
CTAGACG800.00290999621.5506134
CGTATGC6300.021.34562341
CGTTAGC1154.625984E-520.98842427
ATCAACG166000.020.8547613
CCACGTT9100.020.840437
CGAAATT19200.020.83225848
TCAACGC168350.020.5841314
CAACGCA169150.020.5071645
Produced by FastQC (version 0.11.5)