FastQCFastQC Report
Wed 25 May 2016
SRR1033248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013903
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77780.38621522486435544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.038.601271
GTATCAA47700.029.236481
ATCAACG60950.023.4282783
TCAACGC61550.023.0846274
CAACGCA63350.022.428715
TATCAAC62550.022.2931942
AACGCAG65200.021.846176
GTGGTAT13400.021.8008581
TGGTATC13600.019.6882482
ACGCAGA74800.018.85847
CGCAGAG76150.018.4788958
AGAGTAC71600.017.63632611
CAGAGTA75900.017.2718310
GAGTACT45750.017.14742312
TATACTG9800.016.8506285
GCAGAGT79500.016.8359499
GTACATG54150.016.6983661
ATATCGT1250.001977370816.5144396
AGTGCGC1250.001977370816.5144398
TACATGG53550.016.1694452
Produced by FastQC (version 0.11.5)