FastQCFastQC Report
Wed 25 May 2016
SRR1033248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013903
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.039.5104941
GTATCAA54250.029.2911011
TCAACGC68300.023.0220874
ATCAACG68350.023.0052493
TAAGCGC1803.45608E-1022.98842823
GTGGTAT14050.022.619821
CAACGCA69950.022.4790365
TATCAAC70600.022.4186042
AACGCAG72350.021.7333626
TGGTATC15350.019.3192672
CGGCTAT1801.9937033E-719.15702430
ACGCAGA83700.018.6626517
CGCAGAG84750.018.3500568
AGAGTAC82400.017.82763511
GAGTACT55750.017.5046312
GTATAAC4600.017.2721981
CAGAGTA88400.017.12472510
GTACATG54750.017.0986861
GCAGAGT90250.016.88799
CTATACG1452.6970997E-416.6766051
Produced by FastQC (version 0.11.5)