FastQCFastQC Report
Wed 25 May 2016
SRR1033247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12919
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN570.44121062001702915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA353.0137926E-759.2033652
GGTATCA407.5807657E-751.802941
AGGACAG200.00727751251.6005865
TCAACGC407.790786E-751.6005865
CAGGACA200.00727751251.6005864
CCAGGAC200.00727751251.6005863
CAACGCA407.790786E-751.6005866
AACGCAG451.7550265E-645.8671887
TATCAAC451.7550265E-645.8671883
ATCAACG503.62428E-641.2804684
AGTACTT350.001317137939.3147314
ACGCAGA556.975999E-637.52778
CAGAGTA556.975999E-637.527711
AGAGTAC556.975999E-637.527712
CGCAGAG556.975999E-637.52779
GAGTACT400.002537726434.4003913
GCAGAGT652.191471E-531.75420610
Produced by FastQC (version 0.11.5)