FastQCFastQC Report
Wed 25 May 2016
SRR1033246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631162
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN102180.3883455294656885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38000.037.0627141
GTATCAA65250.029.3121681
TATAACG2250.024.7245142
CGCGGTA1602.1846063E-923.73806243
ATCAACG81700.023.4997353
TCAACGC82750.023.1980024
CAACGCA84400.022.7037265
TATCAAC84000.022.4755762
AACGCAG88400.021.7554876
GTGGTAT20400.020.6262841
ACGCAGA99850.019.054377
CGCAGAG100300.018.900288
TGGTATC20200.018.4171622
GTACATG67200.018.1119231
AGAGTAC98350.017.70184911
GAGTACT67100.017.48535212
TACATGG66600.017.2278062
CAGAGTA105600.016.9426710
GCGGTAC2451.573244E-816.91171844
GCAGAGT108400.016.727229
Produced by FastQC (version 0.11.5)