FastQCFastQC Report
Wed 25 May 2016
SRR1033246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631162
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.037.7364651
GTATCAA70350.028.1522851
ATCAACG87150.022.2739983
TCAACGC87150.022.2739984
CAACGCA89000.021.8109995
TATCAAC91800.021.3710942
GTGGTAT20450.021.1270981
AACGCAG92150.021.1028426
TGGTATC19950.019.5295722
CCGACGA900.0057563619.15511350
ACGCAGA106400.018.1145447
GAGTACT69400.017.93548612
CGCAGAG107700.017.8318618
AATACCG2006.0335697E-717.2396015
AGAGTAC105100.017.19071811
AGTACTT73850.016.94812213
GTACATG69100.016.7067341
GTACTTT75400.016.5082614
TATACAG11750.016.4326425
CAGAGTA114900.016.26464810
Produced by FastQC (version 0.11.5)