FastQCFastQC Report
Wed 25 May 2016
SRR1033245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154537
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81280.37725042549745025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.027.0327381
TACACCG1651.200533E-1025.0012255
GTATCAA70350.024.2122121
GCGAACT1154.563843E-521.02448728
ATCAACG87000.019.8780353
TCAACGC87600.019.6998794
CAACGCA88650.019.5053255
TATCAAC86650.019.3721852
AACGCAG92150.018.8768396
GAGTACT60100.018.70673812
AGAGTAC85150.018.57367711
GTACATG55750.018.269441
AGTACTT62550.017.8091213
TACATGG54650.017.5516912
ACGCAGA101450.017.0798077
CGCAGAG101250.016.9437688
GTACTTT65600.016.77654814
TATAACG1250.001848564516.6817362
ACATGGG57450.016.1584053
TAAGCGC1300.0024992115.9453823
Produced by FastQC (version 0.11.5)