FastQCFastQC Report
Wed 25 May 2016
SRR1033245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154537
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49850.027.3852921
GTATCAA76800.022.7706131
ACCGACG800.002909273621.55095344
ATCAACG90350.019.0058673
TCAACGC90450.018.9848544
TATCAAC92450.018.6487452
CAACGCA93200.018.4246795
CCGACGA950.00786085318.14817245
CGATGCG950.00786085318.14817244
AACGCAG96050.017.985686
ACAACGC1753.2268817E-617.7333568
TACACCG1954.6235618E-717.6828355
ATACCGT1602.9658906E-517.2407636
AGAGTAC92450.017.0822511
GAGTACT61550.016.6945512
GTACATG54850.016.2565541
AGTACTT63600.015.93957413
TATATCG1300.002531701515.9145525
ACGCAGA108250.015.7993887
CGCAGAG107950.015.7794118
Produced by FastQC (version 0.11.5)