FastQCFastQC Report
Wed 25 May 2016
SRR1033244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474
Sequences flagged as poor quality0
Sequence length75
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13568521031207598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13568521031207598No Hit
TTTGTAGCCTTTGCCCTTGGTCACCCCGATGACGTCGATCATCTCATCCTGCCCAAACACTTGGTTCACAGGTAC20.13568521031207598No Hit
CACCAAGAAAGACCTGGATGACATCAGCACCAAAACAGGCATCACCCTCAAGAGCTGCCGGAGACAGTTTGACAA20.13568521031207598No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20.13568521031207598No Hit
CATTTGTTGCTGCATTACATTCATTTGCGGTTGCATCATATTTATAGGTAGTTGAGAACCATCAACTTGCTGGTT20.13568521031207598No Hit
GATTAAATCCTTTGAGGATTCTCTTCTCTTTTACCATTTTTCTGCGTGCTCTCACTCTCTCTTTCTCTCTCTAGC20.13568521031207598No Hit
GGTGAGGAACCAGCGGTTGGTATTGGGAAAGGCCTGGCGGAAAGAAGGCTCTAGAACCTGCTTATAGAGCCACAA20.13568521031207598No Hit
GCTGTTGATAAAGGGGCAGGCTTGCGTTATTGGCCTAGATTTTGCTGCAGATTAAATCCTTTGAGGATTCTCTTC20.13568521031207598No Hit
TTATTGGCCTAGATTTTGCTGCAGATTAAATCCTTTGAGGATTCTCTTCTCTTTTACCATTTTTCTGCGTGCTCT20.13568521031207598No Hit
GCCTTAAGGTATGATGTATTTCTGATGGAAGCCATTTTCACATTCATGTTCTTCATGGATTATTTGTTACTTGTC20.13568521031207598No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)