FastQCFastQC Report
Wed 25 May 2016
SRR1033244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCT50.33921302578018997No Hit
CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGA40.27137042062415195No Hit
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTT40.27137042062415195No Hit
CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGG40.27137042062415195No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAAT30.20352781546811397No Hit
CTCTAGCACACATCCTCATGATGGCAGGTGGTTAGCACCAGTCCTACATGGACCAGGATGACACAGCACTATGCT30.20352781546811397No Hit
GTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA30.20352781546811397No Hit
ATTATGTACAGTAGTTAAGTTAGCTCGGGAAGGTGAGTCTCCGCAGCCTGGGTGAACAGTGTGGGATGGAGCCTC20.13568521031207598No Hit
TAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGA20.13568521031207598No Hit
TACAAAACTACTACTCACGACGAGGCAGAAATTCTTCAGGTCCACAGTCTGGATGGATGAAACAAGAGGAGGAAA20.13568521031207598No Hit
ATCACGGGCAAGGAGATTTTCAGCACCATCAGCCACTGGGTCACGTTCAGCGGTGTGATCTGGAAGATGAGTGGC20.13568521031207598No Hit
CTTCCTCCACATCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACT20.13568521031207598No Hit
AGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTA20.13568521031207598No Hit
TTGTAGGGCAATTAAGCCTGGGATTTTAGGGCAGGGATTCTGTACTGTCTCTGGTGGGTAAAGGGTATATACTTC20.13568521031207598No Hit
CATCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTC20.13568521031207598No Hit
GGGATTGGCAGCCCCGCAGACTGAAGTCCTCTGTTCATCCACAGAACAGATCCAAAAAATTAATCCAAGAATCGG20.13568521031207598No Hit
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAG20.13568521031207598No Hit
GTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG20.13568521031207598No Hit
GTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCG20.13568521031207598No Hit
CTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCACCCA20.13568521031207598No Hit
CGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAGGGTGTTCTTCTCCTGCGTCTCCGTTTTCTTCAGCTTGGCC20.13568521031207598No Hit
TGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACA20.13568521031207598No Hit
GTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCG20.13568521031207598No Hit
TCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTCCT20.13568521031207598No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGC20.13568521031207598No Hit
GGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAG20.13568521031207598No Hit
GTGTTGCTGAATATTGTCCACCAGGGAGCCCCGCATTTCCTCTACCACCTTGAAGACCCGTTTAAAGTTGTCAAA20.13568521031207598No Hit
CCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGC20.13568521031207598No Hit
AGTGTACTTGGCGTGCTGGCTGATTTCAATTTTCTTCACCATTTTCCGGAGGGAGGCCCCATAGCGGGTCCCGTA20.13568521031207598No Hit
GGGTTGGTAGATGTGTTGCTAAAAACGCACATGCACGCAACCGAACACCCTTACATTTCTGCAAAATGGTTTAGG20.13568521031207598No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)