FastQCFastQC Report
Wed 25 May 2016
SRR1033242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907111
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75310.3948904914291827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.041.9759561
GTATCAA57700.028.5051571
GTGGTAT13250.023.881611
TCAACGC72850.023.1781124
ATCAACG72750.023.1675873
TGGTATC12900.022.6408632
CAACGCA74500.022.618615
TATCAAC72250.022.6184792
TCTGTCG3850.022.3326248
AACGCAG77050.021.8711916
TATAACG1454.9140726E-721.5813162
CTGTCGC4500.019.8736939
ACGCAGA87750.018.930437
CGCAGAG88650.018.4666758
GAGTACT62250.018.34494812
GTACATG53800.018.226571
TGTCGCC5300.018.17188310
GTCTCGC4800.018.110771
AGAGTAC87700.017.53176511
AGTACTT64200.017.46581613
Produced by FastQC (version 0.11.5)