FastQCFastQC Report
Wed 25 May 2016
SRR1033242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907111
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33450.042.3446921
GACGCGT400.002603469434.48185710
GTATCAA57750.028.7144471
GTGGTAT12950.028.0111391
CGCGCGA500.007756057727.58548721
TGGTATC13450.025.8934382
TTGCGCG750.001999608222.98790418
ATCAACG73750.022.4891853
TCAACGC74500.022.123934
TATCAAC75250.022.0867162
CAACGCA76550.021.5764985
AACGCAG79200.020.8980946
ACGCAGA90450.018.298837
GTACATG51650.018.2601171
CGCAGAG91900.017.9350688
GAGTACT67100.017.4207912
AGAGTAC90950.017.32623311
TACATGG53250.017.2894952
TACCTGG11700.017.093572
GCAGAGT96300.016.9007669
Produced by FastQC (version 0.11.5)