FastQCFastQC Report
Wed 25 May 2016
SRR1033241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017296
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN118590.393034027818285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52550.039.0076331
GTATCAA88100.027.2109381
GTGGTAT22100.022.6381281
ATCAACG108400.022.4942343
TCAACGC110000.022.0677324
CAACGCA111850.021.610515
CGGTACC2401.8189894E-1221.5847845
TATCAAC110100.021.422972
AACGCAG116750.020.7929086
TGGTATC24450.020.6009062
GCGGTAC2901.8189894E-1219.0544744
CGCGGTA2255.47152E-918.41932143
TTAACGG3051.8189894E-1218.10857635
ACGCAGA133800.017.9905937
CGCAGAG134450.017.8016098
GTACATG92300.017.616291
TAACGGC2802.5283953E-1017.25973736
GAGTACT84250.017.10402712
AGAGTAC132050.016.72056211
TACATGG93250.016.5025882
Produced by FastQC (version 0.11.5)