FastQCFastQC Report
Wed 25 May 2016
SRR1033241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017296
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55450.041.12351
GTATCAA93050.027.5508631
GTGGTAT22100.023.2938731
ATCAACG115450.021.7722023
TCAACGC116200.021.6910234
CAACGCA118300.021.3059775
TGGTATC23700.021.0954342
TATCAAC120600.020.9568252
AACGCAG122350.020.6288936
TAGACCG850.00413548620.2824255
ACTATCG1601.3708668E-619.395078
ACGCAGA141250.017.6733517
GTATTAG11150.017.6623171
CGCAGAG141000.017.6068738
GAGTACT88600.017.59060312
AGTACTT91250.016.8530313
AGAGTAC139100.016.60824211
GTACCGT2502.0540028E-816.5504596
GTACTTT93000.016.23929414
GTACATG94700.016.2352051
Produced by FastQC (version 0.11.5)