FastQCFastQC Report
Wed 25 May 2016
SRR1033240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811513
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN111460.39644134670549275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48150.031.219691
GTATCAA77550.027.0119441
ATCAACG93100.022.9772643
TCAACGC94500.022.5976374
CAACGCA97500.021.8317875
TATCAAC95950.021.790962
AACGCAG99700.021.3852986
TAGACCG1154.7228095E-520.9316485
ACGCAGA111850.018.8173077
CGCAGAG112900.018.6121738
GTGGTAT27700.018.0893881
AGAGTAC107800.017.9945611
GAGTACT73300.017.83037612
TCGCCGA1552.299394E-517.75960717
GTACATG70000.017.5476041
AGTACTT76850.016.82740413
TACATGG70650.016.7914852
GCAGAGT121350.016.7788739
ACTACGC2058.0239806E-716.7762938
TGGTATC28350.016.6890952
Produced by FastQC (version 0.11.5)