FastQCFastQC Report
Wed 25 May 2016
SRR1033240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811513
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.032.0124051
CCGCGTA658.685736E-426.5250959
AAGCGCG700.001338248424.63044746
GTATCAA78500.024.4699251
ACGACCG800.002908953221.5516415
TCAACGC96100.019.699234
ATCAACG96200.019.6787553
TATCAAC99300.019.2033162
CAACGCA98350.019.1784385
GCGGTAT1651.8059036E-618.8445641
AACGCAG101550.018.6759666
GTGGTAT27600.018.400761
GAGTACT73250.016.71171612
TGGTATC29400.016.6548752
ACGCAGA116200.016.2323537
GTACATG72000.016.2185381
CGCAGAG116650.016.0810538
AGAGTAC109950.016.02603111
AGTACTT77700.015.71023113
TACATGG73400.015.4091292
Produced by FastQC (version 0.11.5)