FastQCFastQC Report
Wed 25 May 2016
SRR1033239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022435
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77130.3813719600382707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32200.042.3594321
GTCGTAC501.770962E-434.7952461
GTACCGT1101.0913936E-931.2766196
GTGGTAT12900.028.5914421
GTATCAA55850.028.3470691
TGGTATC14450.024.7996542
ATAACGC1251.2999772E-724.771083
ATCAACG70150.022.7073143
TCAACGC70350.022.6905384
CAACGCA71650.022.038775
TATCAAC72250.021.5732942
AACGCAG74600.021.2605536
GTCTCGC4350.020.797161
TATAACG1258.1974E-519.483382
ACGCAGA85650.018.3168147
CGCAGAG85800.018.2045968
GTATAGA4400.018.1884251
TACCGTG1603.0233794E-517.202147
GAGTACT59850.016.843712
AGAGTAC85350.016.80995811
Produced by FastQC (version 0.11.5)