FastQCFastQC Report
Wed 25 May 2016
SRR1033239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022435
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33050.038.35511
GTATCAA57300.029.7783641
GTGGTAT14500.024.7738691
ATCAACG71150.023.8444863
TCAACGC71300.023.6492374
TATCAAC73500.023.2228552
CAACGCA73400.023.066585
TGGTATC14950.022.8345152
AACGCAG77250.022.0508946
ACGCAGA86500.019.573267
CGCAGAG86000.019.566778
TCGCACG900.005751984719.15737322
AGAGTAC85250.018.52591111
GAGTACT61150.017.8760412
GCAGAGT93450.017.6014169
CAGAGTA91650.017.57085610
AGTACTT64500.016.8406713
GTACTTT66850.016.3518314
GTACATG53000.016.2275141
TGCGGTA1503.4717502E-416.1332857
Produced by FastQC (version 0.11.5)