FastQCFastQC Report
Wed 25 May 2016
SRR1033238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065520
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82610.3999477129245904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.040.507761
GTATCAA53450.029.0052621
GTGGTAT13650.026.2297531
TGGTATC13550.025.3946822
ATCGGAT700.001325485924.67059147
ATCAACG65600.023.5883373
TCAACGC65700.023.4443324
CAACGCA66950.023.006615
TATCAAC66950.022.7908672
AACGCAG70450.021.9134966
TCTGTCG5550.020.4445678
GTACATG54750.019.364461
ACGCAGA79650.019.123837
CGCAGAG79800.019.0447968
TACATGG54650.018.8255832
CTGTCGC6100.018.6025629
GAGTACT56650.017.96720312
AGAGTAC80550.017.63084611
ACATGGG56850.017.4805873
GTACAGG10950.017.4597591
Produced by FastQC (version 0.11.5)