FastQCFastQC Report
Wed 25 May 2016
SRR1033238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065520
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32450.040.0122031
GTGGTAT12900.026.5011541
GTATCAA52350.025.7916681
TGGTATC14050.024.2943272
TATACCG902.1362305E-422.9855425
ATCAACG62550.021.3319053
TCAACGC63250.021.041314
CAACGCA64750.020.6071135
TATCAAC65650.020.272092
AACGCAG67650.019.77476
TCTGTCG5850.018.8599328
CAGCGTA1651.8408355E-618.806359
CTGTCGC5700.018.7513629
GTCTCGC3550.017.5090871
ACGCAGA80300.016.6595087
CGCAGAG80150.016.604658
GTACATG58350.016.3929941
GAGTACT52800.016.39025712
TACATGG58100.015.963282
AGTACTT55500.015.34440113
Produced by FastQC (version 0.11.5)