FastQCFastQC Report
Wed 25 May 2016
SRR1033237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634270
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN102230.3880771523040539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39700.038.2527351
GTATCAA68850.028.1140121
ATCAACG85350.023.2887953
TCAACGC85500.023.2439464
GTGGTAT19650.022.8138581
CAACGCA88100.022.440895
TATCAAC86400.022.1996772
TGGTATC19450.021.9738852
AACGCAG91400.021.6691066
GTACATG73850.018.9167771
ACGCAGA104150.018.7526257
CGCAGAG105100.018.5831188
TACATGG74050.017.9250182
GAGTACT66950.017.41259812
AGAGTAC102800.017.3950111
GCAGAGT110150.017.0147829
AGTACTT70000.016.80192613
ACATGGG77850.016.7418443
CAGAGTA108550.016.72701610
CGAACTA2702.8849172E-916.6288729
Produced by FastQC (version 0.11.5)