FastQCFastQC Report
Wed 25 May 2016
SRR1033237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634270
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40700.040.0641561
GTATCAA69150.027.9272421
GTGGTAT17850.025.5472491
TGGTATC17900.023.3090132
ATCAACG83000.022.7663843
TCAACGC83600.022.561744
TATCAAC86200.022.0812362
CAACGCA86000.022.0123025
AACGCAG89650.021.154566
CCGCGTA900.005753732319.1566289
GGACCGT2608.913048E-1118.5671926
ACGCAGA105650.017.8855657
TATAACG1954.623962E-717.6830432
CGCAGAG106700.017.6126088
GTACATG76350.016.8322541
CGGACCG1452.735001E-416.6464485
AGAGTAC106600.016.56167811
CAGAGTA112000.016.25576610
GAGTACT69050.016.12985412
GCAGAGT115500.016.0318589
Produced by FastQC (version 0.11.5)