FastQCFastQC Report
Wed 25 May 2016
SRR1033236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473161
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN96120.3886524168867292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43800.037.628881
GTATCAA69450.025.834171
GTGGTAT20400.024.8851181
TGGTATC21300.023.3395862
ACGACGT953.0495544E-421.80786150
ATCAACG85650.021.4865683
TCAACGC86950.021.1635994
CAACGCA88200.020.8246655
AACGCAG91000.020.3355086
TTCGCGA850.00410044720.31206941
TATCAAC87400.020.166632
TACACCG1601.4031884E-619.3476335
TAGTGCG950.00797105618.1045239
ACGCAGA102150.017.9145167
GTACATG61900.017.8067911
CGCAGAG103000.017.6998868
AGAGTAC99600.017.06114811
TACATGG60650.016.9093912
GAGTACT72000.016.5782112
TATACTG11650.016.2383565
Produced by FastQC (version 0.11.5)