FastQCFastQC Report
Wed 25 May 2016
SRR1033236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473161
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.041.0964851
GTATCAA72550.026.86131
GTGGTAT17750.025.3063981
TGGTATC19100.022.9276312
ATCAACG86650.022.0062183
CGTATAC1103.282276E-521.942913
TCAACGC86800.021.9284634
CAACGCA88850.021.4613275
TATCAAC90400.021.3603552
AGCGAAC1959.404175E-1021.22037527
AACGCAG92600.020.5922136
CGAACTA1851.1848897E-820.50305629
GCGAACT2053.8495273E-818.5031328
ACGCAGA106050.017.7854777
CGCAGAG107350.017.5379758
TATAACG1402.0922587E-417.2408582
GTACATG64000.016.5746271
AGAGTAC106700.016.54638711
GAGTACT74000.016.35584612
CCGACGT1300.002482975115.96110366
Produced by FastQC (version 0.11.5)