FastQCFastQC Report
Wed 25 May 2016
SRR1033235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429434
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56950.3984094403798986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG350.001296185239.743732
GCGTAAG652.1983618E-532.1018451
TAATCGC553.3089612E-431.27976810
CGATAGA1001.48411345E-830.97677216
CCGATAG953.2726348E-728.98309515
TGTCGCA500.007718310727.61259856
TTTCGAC809.531466E-525.88772433
GCGCCGT700.0013271824.66454327
TACGAAC700.00132991124.65584463
CGGATTA700.001330731324.65323659
GTGCGTA1001.5967657E-524.0854211
CGCAATG750.00198348923.01942822
CGAGTCG800.00288913621.57614767
GACTCGT800.002892686321.57158357
GTGTCCG1605.8851583E-821.5048411
GATAGAC1356.966022E-620.39622917
TCGACGC850.004109776620.30338135
GGGTCGT850.004188441720.2370037
CGAACCA1055.9954764E-419.72815530
CTTAGAC3400.019.2285333
Produced by FastQC (version 0.11.5)