FastQCFastQC Report
Wed 25 May 2016
SRR1033235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429434
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA102980.7204250073805436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG400.002602129734.4848954
TATGCCG4950.032.04544443
CGGTTCG450.00463377630.65216324
GTCCGAC450.00463377630.65216324
TAGTGCG500.00765334427.6604865
CGCATTA658.682084E-426.5259140
GCGCATT658.682084E-426.5259139
CTACGGT658.682084E-426.5259129
CTCGTAT5750.025.78779839
GACGTAT1101.152237E-625.09308464
TCGTATG6200.025.02847940
ACCGTCC1251.2707824E-724.828258
GGACGTA1001.5697482E-524.13857863
CGTATGC6450.024.05838241
TGCCGTC6500.023.8733245
TCTCGTA6300.023.53648238
TTATCGA902.133532E-422.98912235
CGAATCG750.001998749322.98912239
ATCTCGT6500.022.81228337
GATCTCG6750.022.47825236
Produced by FastQC (version 0.11.5)