FastQCFastQC Report
Wed 25 May 2016
SRR1033234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622373
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN102780.39193509085092015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37750.036.8806761
GTATCAA66050.030.1424881
ATCAACG83650.023.8519943
TCAACGC83600.023.8200864
CAACGCA85400.023.3587445
TATCAAC86000.022.863722
AACGCAG88300.022.5149756
GTGGTAT17450.021.5419081
CGTATCG1004.4200363E-420.64178310
ACGCAGA98900.019.9287157
CGCAGAG99100.019.5761138
GAGTACT66800.018.84953112
TGGTATC18100.018.8427542
AGAGTAC99250.018.37135911
GTACATG67250.017.804181
AGTACTT70150.017.80190813
GCAGAGT109350.017.3666579
CAGAGTA107950.017.33693110
GTACTTT73150.017.07542614
TACATGG68300.016.814742
Produced by FastQC (version 0.11.5)