FastQCFastQC Report
Wed 25 May 2016
SRR1033234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622373
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40800.037.8625951
GTATCAA68200.028.9344181
GTGGTAT16700.025.4537411
ATCAACG83000.023.0979373
TCAACGC83000.023.0563934
CAACGCA85550.022.3691485
TATCAAC86600.022.297012
TGGTATC18700.021.7578952
AACGCAG89050.021.6061176
TACGTCG850.00413492120.2827845
TAGACCG900.005754880619.1559625
ACGCAGA102300.018.7402617
CGCAGAG102450.018.477238
GAGTACT66900.017.93650812
GTACATG71800.017.6646331
GTACCGG2359.296855E-917.6472231
AGAGTAC102550.017.41722111
TACATGG70150.016.9085852
GCAGAGT109350.016.9017149
CAGAGTA108750.016.61448110
Produced by FastQC (version 0.11.5)