FastQCFastQC Report
Wed 25 May 2016
SRR1033233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327083
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89990.38670730695896965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40550.037.4839251
GTATCAA70050.028.79881
ATCAACG87550.022.913753
TCAACGC88500.022.6623944
TATCAAC89150.022.1591472
CAACGCA91050.021.9143495
CCGAACG800.002887137221.5794230
AACGCAG94600.021.056986
GTGGTAT19050.019.3537621
GTACATG79550.019.2383141
TACATGG77950.018.4718462
ACGCAGA108650.018.3047227
CGCAGAG108800.018.1533748
AGAGTAC102050.017.90540111
CCGATCG1351.6091642E-417.8429579
ACATGGG82850.017.6929743
GAGTACT64250.017.62078712
TGGTATC20850.017.3481562
CGACGAC1200.001475205217.25380933
TAGCGCC1603.018319E-517.2057089
Produced by FastQC (version 0.11.5)