FastQCFastQC Report
Wed 25 May 2016
SRR1033233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327083
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44050.038.1092831
GTATCAA73450.027.5108811
GTGGTAT19600.023.0863721
TGGTATC19450.022.6935442
ATCAACG91400.021.8446143
TCAACGC91600.021.8345644
CAACGCA93300.021.3997615
TATCAAC93450.021.3654122
AACGCAG96500.020.7973356
ACGCAGA112300.017.7484367
GTACATG80100.017.5941431
GAGTACT69600.017.53904712
CGCAGAG113550.017.4619488
CCGATCG1200.001482119117.24177618
TACATGG79850.017.1446082
AGTACTT71700.017.0253513
AGAGTAC111400.016.74650211
TCGAACG1250.001947616116.55210553
CAGAGTA118350.016.22926910
GTACTTT75900.016.08323714
Produced by FastQC (version 0.11.5)