FastQCFastQC Report
Wed 25 May 2016
SRR1033232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383621
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN93200.39100175741025944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.036.750121
GTATCAA58750.028.9499321
ATCAACG72250.023.7077353
TCAACGC73000.023.4597074
TATCAAC74000.023.0294282
CGCGTAA750.00202895222.93065610
CAACGCA75100.022.8037095
AACGCAG77750.022.0278726
GTGGTAT16300.021.1248761
ATAACGC2001.3315002E-920.6371563
TGGTATC16150.020.4583132
TTACGGT1206.551526E-520.0600914
GTACATG63200.018.8215581
TATAACG2053.490095E-818.6619742
ACGCAGA91200.018.5156697
TACATGG62200.018.2282922
CGCAGAG92350.018.1737618
GAGTACT61650.017.74196812
AGTACTT64700.017.01156613
AGAGTAC91150.016.98101411
Produced by FastQC (version 0.11.5)