FastQCFastQC Report
Wed 25 May 2016
SRR1033232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383621
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37650.038.454771
GTATCAA60550.026.022671
GTGGTAT17150.025.3867781
TGGTATC18600.022.9873452
TCAACGC74050.020.9540274
ATCAACG74150.020.8792673
CAACGCA76750.020.2168835
TATCAAC76950.020.2091462
AACGCAG80150.019.4022946
GTACATG65350.018.1892171
TACATGG66900.016.9570332
ACGCAGA93050.016.8236247
CGCAGAG93650.016.5685628
GTCTCGC5600.016.0430321
ACATGGG67650.016.0044923
TACACCG3058.6765795E-1015.8273535
GAGTACT63150.015.56150212
AGAGTAC95500.015.30884911
CCTATAC6150.015.1380063
ACACCGT3201.711669E-915.08544356
Produced by FastQC (version 0.11.5)