FastQCFastQC Report
Wed 25 May 2016
SRR1033231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4278128
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165470.386781321175991No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA61130.14288960031116413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG1852.7326132E-718.5923948
GTACATG164300.016.6993141
GGTATCA158600.016.0058541
GAGTACT78400.015.75147312
TACATGG170700.015.6429922
AGTACTT78750.015.63760113
ACCGTAG1350.003310909315.287088
GTATCAA199900.015.2735821
ACATGGG182800.014.865113
GCGTAAT3403.954483E-914.21699753
AGAGTAC176050.014.18545711
GTACTTT87800.014.14744314
TCAACGC230100.013.4367414
CGAATCG1550.00808030913.36355364
TATACTG12100.013.3586745
ACGCGCT2851.5987243E-613.32652244
AACGCAG231050.013.3228946
ATCAACG232550.013.282423
CAACGCA232900.013.2752025
TATCAAC228700.013.2113052
Produced by FastQC (version 0.11.5)