FastQCFastQC Report
Wed 25 May 2016
SRR1033231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4278128
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG400.002604096234.48113643
GACGCGT553.2689658E-431.34648910
GACGTAT1052.29233E-522.98742530
TATGCCG7650.019.38155443
TAAGCGC2406.148184E-1018.677523
ACGCGTT950.00786206918.14817811
TATACCG3103.6379788E-1217.7967155
CTCGTAT8150.017.34633839
ACGTATG1402.0929764E-417.24056831
GTACATG168250.017.1483551
GAGTACT78300.016.77837412
AGTACTT79200.016.457113
GGTATCA158200.016.0535811
TACATGG173150.016.030792
TCGTATG9350.015.857635540
CGTATGC9600.015.44467541
GCGTAAG1350.003256811515.32494943
GTATCAA199500.015.224291
TATAACG2503.6985148E-715.17172
GTACTTT86650.015.121737514
Produced by FastQC (version 0.11.5)