FastQCFastQC Report
Wed 25 May 2016
SRR1033230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227237
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85010.3816836735381102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.039.3224751
GTATCAA60250.028.9829121
ATCAACG75450.023.5189363
TCAACGC76150.023.4345194
CAACGCA78000.022.8787025
TATCAAC77550.022.3792082
AACGCAG81250.022.0078586
GTGGTAT16650.021.9366551
TGGTATC16150.021.320072
GTACATG62150.020.373031
ACGCAGA90950.019.2843277
CGCAGAG91000.019.1229958
TACATGG62600.018.889942
CGAACTA2053.7827704E-818.53116629
ACATGGG65700.017.9537813
CGATCTA4050.017.90113469
AGAGTAC89100.017.79305311
CAGAGTA94450.017.1492910
GCAGAGT97700.017.0719919
GAGTACT60200.017.02342612
Produced by FastQC (version 0.11.5)