FastQCFastQC Report
Wed 25 May 2016
SRR1033230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227237
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35450.040.1426851
GTATCAA61900.029.0160391
TATAACG1602.220986E-923.7048362
ATCAACG79750.022.2226923
TCAACGC80400.022.0430344
GTGGTAT15350.021.8267351
TATCAAC82000.021.7390692
CAACGCA82200.021.4764445
AACGCAG85650.020.6516276
TAACGCA2800.019.7027234
ATAACGC2303.4742698E-1019.488563
TGGTATC16950.019.324942
GTACATG62600.018.5391731
ACGCAGA98400.018.010777
CGCAGAG98800.017.763368
GAGTACT68000.017.23988212
TAAGCGC1402.0930455E-417.23988223
TACATGG62550.017.1985382
AGAGTAC98200.016.78342611
AGTACTT70200.016.5522513
Produced by FastQC (version 0.11.5)