FastQCFastQC Report
Wed 25 May 2016
SRR1033229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853782
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72660.3919554726499664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20520.11069262728842981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT450.00460440530.69249248
CCGGTTA703.914228E-529.50234419
CCGTGTA756.283044E-527.5235710
GCGACAC658.642683E-426.54707762
GGTATCA75450.026.3241421
GACGAAC1101.1496068E-625.1004258
GTATCAA118850.024.9647481
CGCGACA700.001331629724.65085661
CGTTACT700.001352671724.58461817
GGTCCGT851.4725374E-424.2822077
TCTCGCG750.001989086223.00871858
CTCAACG952.9034758E-421.9663222
CTCGCGA800.002894173121.57008659
TCGCGAC1154.5930592E-521.00681760
AACGCAG147350.020.6136366
TCAACGC149700.020.2665144
CAACGCA149750.020.1678665
ATCAACG150900.020.1547663
CCGAACG1056.138456E-419.65969313
AGTACTT91100.019.60034613
Produced by FastQC (version 0.11.5)