FastQCFastQC Report
Wed 25 May 2016
SRR1033229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853782
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATACG350.001331808439.525858
CTTAACG400.002604263434.4796621
TATAACG652.3316643E-531.82738146
AACTCGT450.00463676930.6485932
ATACGAG605.442209E-428.7330556
GGTATCA82350.028.439791
AACGAAT500.00775841327.5837338
TGTGTCG500.00775841327.5837310
AGCGAAC5550.025.47146227
ACCGATC851.4495535E-424.3385878
TATCGGT851.4495535E-424.33858724
GCGAACT5950.023.75909428
GTAAGCG902.1355487E-422.98644346
TATGCCG10200.022.64840743
GCCGTCT10200.022.64840746
GTATCAA121550.022.479251
GTATCCG1103.2370168E-521.981921
CTCGTAT10200.021.97233439
GTACCGT953.0788494E-421.7766286
CGTTCGG953.0788494E-421.77662824
Produced by FastQC (version 0.11.5)