FastQCFastQC Report
Wed 25 May 2016
SRR1033228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630341
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63940.39218789198088005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.035.870871
CGACCGT500.007682827.63893321
GACCGTC1057.912804E-726.1985787
GTATCAA52150.025.659191
GGACCGT1151.749082E-623.9189686
TCTGTCG4400.022.663268
ATCAACG64450.020.5934013
TCAACGC64800.020.5333334
CAACGCA65550.020.2459475
AACGCAG66650.020.0677996
TATCAAC66300.019.9688572
GTACATG37050.019.4186331
GTGGTAT16300.018.3378071
CGTAGAC1702.5126956E-618.20363
TACATGG37150.018.052622
TACCTGG9850.017.9918442
TGGTATC15650.017.7630462
ACGCAGA74850.017.6866687
CTGTCGC5450.017.6660169
CGCAGAG76000.017.419048
Produced by FastQC (version 0.11.5)