FastQCFastQC Report
Wed 25 May 2016
SRR1033228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630341
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36750.032.8899081
TATAACG802.7755177E-630.1694132
TCTGTCG4800.025.8594998
GTATCAA55600.022.8573021
CTGTCGC5350.022.556579
TGTCGCC6300.020.24976310
GTACCTG10750.019.9175171
CGATATG900.005640015519.22239165
ATCAACG66050.019.0014763
TACCTGG10550.018.955462
TACACCG1108.2720025E-418.8069065
TCAACGC66450.018.7833214
GGACCGT1301.18685224E-418.5657926
TATCAAC68100.018.5307432
CAACGCA67650.018.5011045
GTCTCGC3950.018.3600641
AACGCAG70450.017.8147286
GTCGCCC6800.017.74671411
TCGCTCT4750.017.4211354
TAGACAG5350.017.4007845
Produced by FastQC (version 0.11.5)