FastQCFastQC Report
Wed 25 May 2016
SRR1033227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870368
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34050.39121383139085997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG803.092282E-1143.495792
GCGTACC350.001344575739.44677764
GGTATCA17900.037.3303071
TATCACG652.1888967E-532.1199652
ATAACGC1359.094947E-1230.5779023
GTACCTG7800.029.4484731
GTATCAA30800.029.0399481
GTACACG854.1354306E-628.661081
TACCTGG7800.028.5510832
GTATAAC1750.027.842191
ACGCTAC756.135125E-527.61755441
AGCGTAC500.007720832227.60953963
TCTGTCG2800.027.0271178
TAACGCA2100.024.5686874
ATCAACG38450.023.5298753
TCAACGC38450.023.5271514
TATCAAC38250.023.0158122
CAACGCA39250.022.9599845
GTGGTAT8300.022.6427481
TGGTATC8050.022.4773252
Produced by FastQC (version 0.11.5)