FastQCFastQC Report
Wed 25 May 2016
SRR1033227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870368
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.10650667303944998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG900.053.6333772
ATAACGC1000.048.270043
GGTATCA17600.041.0352751
TACCTGG7600.038.1079252
GTACCTG7950.035.2080231
GTCTCGC2650.032.6000251
GTATCAA31400.030.0439281
GTATAAC2000.027.6448171
TAACGCA1950.026.5220034
CGAATTA809.595872E-525.86043515
CTGTCGC2900.024.9686999
TCTGTCG2950.024.5440888
GTACACG851.4271047E-424.3924871
TCGCTCT3400.024.3378354
ACCTGGG11600.024.0755753
GTGGTAT7150.023.681751
TGGTATC7300.023.143172
TATCAAC40700.023.1269252
ATCAACG40650.023.070553
TCAACGC41150.022.7064424
Produced by FastQC (version 0.11.5)