FastQCFastQC Report
Wed 25 May 2016
SRR1033226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981135
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN119620.40125656838754364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72750.026.1750871
GTATCAA107600.022.2508981
ATCAACG127500.018.9640373
GTACATG74750.018.873631
TCAACGC128500.018.7603934
ACCGTCG1301.2104421E-418.5190358
GAGTACT83250.018.46753512
CAACGCA129950.018.4187435
TATCAAC130150.018.1796022
TACATGG74700.018.0464822
AACGCAG133550.017.9737476
AGAGTAC121500.017.72081611
ACATGGG79550.017.3376353
AGTACTT88700.017.21621313
GCGTATC1855.0922336E-616.9048521
GTACTTT92700.016.4769514
ACGCAGA147200.016.214947
CGCAGAG148750.015.884138
TAGACCG1300.00257750515.872125
GGACCGT2654.3462933E-815.5726466
Produced by FastQC (version 0.11.5)