FastQCFastQC Report
Wed 25 May 2016
SRR1033226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981135
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72250.025.779441
TTACGCG700.00131509124.704469
GTATCAA102850.021.1669771
CGAACGA850.004134348620.28337942
TACACCG2102.3683242E-919.7038525
GTACATG75800.017.961791
ATCAACG121350.017.6173733
TCAACGC121900.017.5095984
GAGTACT81900.017.43033212
GTTACGC1602.8903365E-517.2930868
CAACGCA124150.017.2200415
TATCAAC125950.017.1108282
TACATGG76950.016.848782
AACGCAG128700.016.6380426
AGAGTAC120200.016.52369711
AGTACTT86200.016.52083613
ACATGGG80200.015.5640793
GTACTTT91600.015.3586814
ACACCGT2959.5187715E-915.1953446
GTACGAC1851.02680184E-414.9110233
Produced by FastQC (version 0.11.5)