FastQCFastQC Report
Wed 25 May 2016
SRR1033225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381929
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN133160.3937397857849766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.040.793571
GTATCAA112500.028.7713031
GCGAACT7100.025.289628
GTGGTAT25850.023.6708851
ATCAACG139950.023.3769263
TCAACGC142800.022.8815164
CAACGCA144800.022.5417185
TGGTATC26150.022.4656332
CGCGGTC1854.820322E-1022.38883832
TATCAAC145750.022.061592
CGAACTA8200.021.89676929
GTACATG147850.021.657121
AACGCAG151950.021.6184396
AAAGCGA8450.021.25527825
TACATGG142750.020.9423772
CCAACGG8750.020.90784663
ACGGAAC8600.020.87270566
GGTCCGT1507.5544995E-720.638257
CAACGGA8950.020.4418564
CGGAACA8850.020.28308367
Produced by FastQC (version 0.11.5)