FastQCFastQC Report
Wed 25 May 2016
SRR1033225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381929
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66250.039.0079571
GTATCAA120050.027.282471
CCGAAAT8200.023.96759247
GTGGTAT29850.022.801291
CGCGGTA4400.022.7252543
ATCAACG147950.022.0231913
TCAACGC149050.021.8375264
CGAAATT8850.021.8176648
TGGTATC29300.021.7704522
TCGCCCC8650.021.52521138
GCGAACT6100.021.47947128
CAACGCA152350.021.387145
TATCAAC153250.021.306542
ACGGCCG4550.021.21858838
AACGCAG158000.020.731466
CGGTACC4850.020.61672445
GCTCGGA9750.020.51099811
GCTCCGA9900.019.85223829
TAACGGC5050.019.80051236
CGCGGTC1409.399853E-619.70268232
Produced by FastQC (version 0.11.5)