FastQCFastQC Report
Wed 25 May 2016
SRR1033224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205249
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86750.3933796138213871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38350.040.184391
GTATCAA63050.026.9248771
ATCAACG78900.021.4950283
TCAACGC79800.021.3358974
GTGGTAT16700.021.0389421
TATCAAC80500.020.6975332
TATAACG1356.3605457E-620.6127132
CAACGCA82750.020.4921515
TACGCGA850.004103979620.3089344
AACGCAG83950.020.2825646
GTACATG69750.018.2040351
GTCTAGC2156.003211E-817.7980841
GAGTACT64200.017.52228212
ACGCAGA96700.017.4304247
GTATTAG5800.017.3935811
TGGTATC19350.017.2571562
AGAGTAC93450.017.22838211
TACATGG70250.017.082512
CGCAGAG99700.017.0094438
AGTACTT66450.016.87720713
Produced by FastQC (version 0.11.5)