FastQCFastQC Report
Wed 25 May 2016
SRR1033224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205249
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40600.035.2208331
GTATCAA64500.026.7218281
TATAACG1401.39807526E-824.6285252
GTGGTAT19750.024.4842191
CGTATAC800.002910074321.549963
ATCAACG79950.021.218423
TCAACGC80000.021.118964
TATCAAC81050.020.9304472
CAACGCA82000.020.6038635
TGGTATC22500.020.5347182
AACGCAG83600.020.2920176
GTATGCG900.005755365419.155523
ACGCAGA96850.017.4802767
GTACATG67300.017.2444571
CGCAGAG99100.017.0833978
AGAGTAC93450.017.04625911
ATAACGC1855.4817483E-616.7740233
GAGTACT63450.016.6286212
TACATGG69150.016.404772
CAGAGTA100300.016.15709910
Produced by FastQC (version 0.11.5)