FastQCFastQC Report
Wed 25 May 2016
SRR1033223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126807
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83760.3938298115437837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33800.040.552611
GTATCAA59250.030.0034921
GTGGTAT14100.025.906541
TGGTATC14150.023.844152
ATCAACG76250.023.5463753
TCAACGC78000.022.971284
CAACGCA80450.022.2289715
TATCAAC78650.022.0682742
AACGCAG83550.021.3233856
CGACGTT1409.297728E-619.7269567
GTATAGG6000.019.7136441
GTCTAGA4350.019.1938111
ACGCAGA95350.018.648897
CGCAGAG95950.018.3888918
GTACATG56850.017.9298381
TACATGG56100.017.3604812
AGAGTAC94300.016.81484811
GCAGAGT101600.016.6223139
CAGAGTA99550.016.41179310
GAGTACT68800.016.29794512
Produced by FastQC (version 0.11.5)