FastQCFastQC Report
Wed 25 May 2016
SRR1033223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126807
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33900.043.4135671
GTATCAA59850.028.919391
GTGGTAT14200.027.735251
TGGTATC14800.024.9281122
GTCGTAC902.1074833E-423.031611
TATGCGT750.002000307722.9866714
ATCAACG76600.022.371493
TCAACGC77400.022.2293594
CAACGCA79500.021.6421665
TATCAAC79800.021.4743882
AACGCAG82400.020.9641786
GTCTCGC3600.020.152661
CCGTACC1108.2720415E-418.80727648
CAACCGT1301.186889E-418.5661586
ACGCAGA95200.018.036817
GTACATG56050.017.813031
CGCAGAG95350.017.7914668
TAGACTG6250.017.6537635
GAGTACT69900.017.60995912
TCTGTCG4150.017.4477148
Produced by FastQC (version 0.11.5)